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Dr Anja Kaczmarczyk

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Dr Anja Kaczmarczyk
Position:
Research Scientist
Phone:
(+61 8) 9480 3969
Fax:
(+61 8 9480 3641
Email:
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Research themes:

  • Micropropagation
  • In vitro culture
  • Cryogenic storage
  • Endangered plants
  • Ex situ conservation
  • Conservation and propagation of Australian plants.

Academic background:

Anja studied biology at the University of Leipzig in Germany with main focus on plant physiology, ecology, botany and biochemistry. She finished her studies with a Diplom (master degree) in Biology. Later on she did her doctorate at the faculty of natural sciences in the department of life sciences at the Martin-Luther University Halle-Witttenberg, Germany in 2008. The experimental work of the dissertation was done at the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) in Gatersleben, Germany. She studied mainly the physiological, biochemical, histological and ultrastructural aspects of cryopreservation in meristematic tissue of potato shoot tips. In October 2009 she jointed Curtin University of Technology and the BGPA for a Postdoc project on cryopreservation of Western Australian plants.

Project:

Title: Development of cryopreservation for high value provenance collections of recalcitrant plant species used in post-mining restoration

University: Curtin University of Technology, Faculty of Health Sciences, School of Biomedical Sciences

Supervisors: Dr Ricardo Mancera, Dr Eric Bunn, Dr Shane Turner

Industry partner: This project is in association with Alcoa-Australia and Worsley Alumina.

The specific aims of the project are:

  • Design various plant cell membrane model systems through molecular modelling simulations to investigate the molecular mechanism of cryo-induced cell damage (and its prevention by typical cryoprotective agents) as a function of membrane composition.
  • Characterise the biophysical properties of cell membranes of ‘model’ plant species to determine how cryoprotective agents protect them from freezing and dehydration damage.
  • Test these model membrane systems against real-time cryogenic experiments to provide data on key cell membrane properties.
  • Test these model membrane systems against a range of plant taxa undergoing cryogenic storage to determine their robustness as a predictive tool for cryogenic performance in plant tissues.
  • Develop the predictive model systems to allow fine-tuning of cryogenic protocols prior to testing on new species.
  • Validate these protocols with the cryopreservation of recalcitrant and endangered plant species as a means to maintain long-term collections of key genotypes that the partner organisations (Alcoa World Alumina and Worsley Alumina) require for environmental rehabilitation.

Publications:

Refereed publications:

  • Kaczmarczyk, A., Shvachko, N., Lupysheva, Y., Hajirezaei, M-R. and E.R.J. Keller (2008) ‘Influence of alternating temperature preculture on cryopreservation results for potato shoot tips’, Plant Cell Reports 27, pp 1551-1558
  • Kaczmarczyk, A., Rutten, T., Melzer, M. and E.R.J. Keller (2008) ‘Ultrastructural changes associated with cryopreservation of potato (Solanum tuberosum L.) shoot tips’, CryoLetters 29, pp 145-156
  • Keller, E.R.J., Kaczmarczyk, A. and A. Senula (2008) ‘Cryopreservation for plant genebanks – a matter between high expectations and cautious reservation’, CryoLetters 29, pp 53-62

Book chapters:

  • Keller, E.R.J., Senula, A. and A. Kaczmarczyk (2008) ‘Cryopreservation of herbaceous dicots’. in Plant Cryopreservation: A Practical guide, ed. B.M. Reed, Springer, New York, pp 281-332

PhD thesis:

  • Kaczmarczyk, A. (2008) ‘Physiological, biochemical, histological and ultrastructural aspects of cryopreservation in meristematic tissue of potato shoot tips’, Verlag Dr. Köster, Berlin, ISBN 978-3-89574-694-9

Conference publications:

  • Kaczmarczyk, A., Grübe, M. and E.R.J. Keller (2009) ‘Present state of potato cryopreservation – new aspects reviewed’, Society of Low Temperature Biology, Meeting Abstracts, University of Copenhagen, Denmark 2008, CryoLetters 30, pp 81-82
  • Kaczmarczyk, A., Senula, S., Grübe, M. and E.R.J. Keller (2009) ‘DSC – Anwendung in der Kryokonservierung vegetativer pflanzengenetischer Ressourcen’ in Anwendungen der Thermischen Analyse, Mikrokalorimetrie und Rheologie im Bereich der Pharmazie, Biotechnologie, Lebensmitteltechnologie und Kosmetik - Würzburger Tage 2008, ed. W. Kunze, TA Instruments / Waters GmbH, pp 15-29
  • Kaczmarczyk, A., Grübe, M. and E.R.J. Keller (2008) ‘Influence of alternating cold preculture on cryopreservation results for potato shoot tips’, Society of Low Temperature Biology, Meeting Abstracts, University of Derby, UK, 12-14 September 2007, CryoLetters 29, p 79
  • Keller, E.R.J. Senula, A., Kaczmarczyk, A., Büchner, D. and C. Zanke (2008) ‘Different protocols – different situations – different genotypes from the research laboratory to application in genebanks. Some subjects of discussion’, Cryopreservation of crop species in Europe, CRYOPLANET, Cost Action 871, 20-23 February 2008, Oulu, Finland, Agrifood Research Working Papers 153, p 61
  • Kaczmarczyk, A., Grübe, M. and E.R.J. Keller (2007) ’Cryopreservation of potato: New Results from the IPK Gatersleben. Germany’, Memories of the International Congress of Plant Biotechnology and Agriculture (BioVeg2007) CD ROM. Centro de Bioplantas,
    Ciego de Avila, Cuba, 7-11 May, pp 1-8
  • Keller, E.R.J., Kaczmarczyk, A. and K.J. Dehmer (2007) ‘On the impact of cryopreservation on genetic resources conservation of the two most advanced temperate crops – potato and garlic’, Proc. 18th EUCARPIA Genetic Resources Section Meeting, 23-26 May, 2007, Piestany, Slovak Republic, p 39
  • Kaczmarczyk, A., Keller, E.R.J., Shvachko, N.A. and Y.V. Lupysheva (2007) ‘Optimization of cryopreservation conditions for two potato accessions (Solanum tuberosum ‘Desiree’ and S. acaule)’, II Vavilov International Conference, Genetic Resources of cultivated plants in XXI century, St. Petersburg, Russia, 26.-30 November, pp 213-215
  • Kaczmarczyk, A., Keller, E.R.J., Melzer, M. and T. Rutten (2006) ’Ultrastructural analysis of potato cryopreservation using the droplet method’, Annual Scientific Meeting Society of Low Temperature Biology, Hamburg, 24.-27. June, Cryobiology 53, p 426
  • Keller, E.R.J., Senula, A. and A. Kaczmarczyk (2006) ‘Development and organization of the Gatersleben cryobank of potato, garlic and mint – maintenance, safety and logistics’, Annual Scientific Meeting Society of Low Temperature Biology, Hamburg, 24.-27. June, Cryobiology 53, p 401

Conference proceedings:

  • Kaczmarczyk, A., Zanke, C., Senula, A., Grübe, M. and E.R.J. Keller (2009) ‘Thermal analysis by differential scanning calorimetry for cryopreservation of potato shoot tips’, 1st International Symposium on Cryopreservation in Horticultural Species, Leuven, Belgium, 5.-8. April 2009 (Poster presentation, manuscript submitted)
  • Kaczmarczyk, A., Witzel, K., Matros, A. and H.-P. Mock (2007) ‘Investigations on cryopreservation of potato shoot tips – new results using a proteomics approach’, Institut’s Day IPK, Gatersleben, Germany, 22.-23. October (Poster presentation)
  • Kaczmarczyk, A., Hincha, D.K. and E.R.J. Keller (2006) Determination of cold tolerance of wild- and cultivated potatoes to explore presumable links to cryopreservation results. 2nd ISC IPK student conference Gatersleben, 29 May- 1 June (Poster presentation)